A review of one-step fluorescent cyanoacrylate techniques

A review of recent research in the use of one-step fluorescent cyanoacrylate techniques is presented. Advantages and disadvantages of such techniques in comparison to two-step processes are discussed. Further studies and new experimental data are presented to aid this review: three one-step cyanoacrylate products (Lumicyano, PolyCyano UV and PECA Multiband) containing a fluorescent dye were tested to evaluate their effectiveness in developing latent fingermarks on polyethylene bags by means of a pseudo operational trial. The results were compared to the traditional two-step process of cyanoacrylate fuming followed by staining with ethanol-based basic yellow 40 (BY40). The study was conducted using sequential treatments of an initial fuming cycle, a second cycle and finally BY40 staining. LumicyanoTM and PolyCyano UV performed similarly before BY40 staining, with both providing good contrast and visibility under fluorescence. PECA Multiband, however, did not develop as many fingermarks and proved to be problematic for the fuming cabinet. Subsequent BY40 staining of fingermarks developed by all three one-step processes enabled the visualisation of new fingermarks

Disruption of Imprinting and Abnormal Growth in Hybrids

Genomic imprinting is a mechanism that regulates the expression of one copy of a gene differently from the other. Disruption of genomic imprinting has been linked to growth-related disease and tumor formation in humans, as well as growth effects in hybrids between many mammal species. Here we use hybrid dwarf hamsters as a model to link the evolution of gene regulation to development in mammals. Previous work has identified growth-related genes that show abnormal imprinting in placental tissues of dwarf hamsters, and whose expression levels are significantly lower in hybrids than in either parent species. These observations have led to the hypothesis that these genes are under-expressed growth repressors, however, the underlying mechanism of this remains unknown. This study examines whether disruption of DNA methylation, a common form of imprinting, could be causing the under-expression of these genes. Genes tend to be activated when not methylated, and silenced when highly methylated. I have identified candidate promoter regions, regulatory regions upstream of a gene, of a subset of these miss-expressed genes to test for differential methylation patterns between the overgrown hybrids and the parent species. I performed bisulfite sequencing on these gene regions, which allows me to quantify patterns of differential methylation. I am using these data to test the hypothesis that changes in DNA methylation underlie the disruption of gene expression in overgrown hybrids. The results will allow us to connect specific regulatory mechanisms to these striking phenotypes, giving us an increased understanding of how the evolution of gene regulation contributes to speciation and diverse growth-related diseases in humans

The effect of mark enhancement techniques on the presumptive and confirmatory tests for blood

An investigation into the effects of physical and chemical enhancement on subsequent presumptive and confirmatory tests for human blood is presented. Human blood was deposited onto porous (white 80gsm paper and brown envelope) and non-porous (tile and linoleum) substrates in a depletion series (30 depletions on non-porous and 20 on porous) and subjected to three ageing periods; 1, 7 and 28 days. A number of enhancement techniques were tested [fluorescence, black magnetic powder (BMP), iron-oxide black powder suspension (PS), cyanoacrylate (CA) fuming, acid violet 17 (AV17), acid yellow 7 (AY7), ninhydrin, DFO and Bluestar Forensic Magnum (BFM) luminol] to evaluate their potential effects on subsequent presumptive and confirmatory tests. AV17 and Bluestar provided the best enhancement and fully enhanced all depletions in the series. The sensitivity of the Kastle-Meyer (KM) (presumptive), Takayama and RSID-Blood tests (confirmatory) was initially investigated to determine the range of detectable depletions. The KM test detected all depletions, whereas the Takayama test detected up to depletion 6 and RSID-Blood detected up to depletion 20 (paper), 10 (envelope), 15 (tile) and 9 (lino). The abilities of these tests to detect blood after enhancement were then observed.A number of techniques resulted in little to no effect on any of the blood tests, whereas adverse effects were observed for others. Ninhydrin and CA fuming caused weak but instantaneous positive KM results whereas methanol-based AV17 and AY7 delayed the reaction by as much as 1 min. The Takayama test was not very sensitive, therefore, its performance was easily affected by enhancement and negative results were often observed. RSID-Blood tests were largely unaffected by chemical enhancement although a drop in positive results was observed for some of the techniques when compared to positive controls.Using a standard procedure for DNA extraction, all the tested blood samples (before and after enhancement) gave a detectable quantity of DNA and were successfully profiled. Out of the 45 samples processed for DNA profiling, 44 gave full profiles, while the remaining showed allele drop out in one or two loci

Erythrocyte enrichment in hematopoietic progenitor cell cultures based on magnetic susceptibility of the hemoglobin

Using novel media formulations, it has been demonstrated that human placenta and umbilical cord blood-derived CD34+ cells can be expanded and differentiated into erythroid cells with high efficiency. However, obtaining mature and functional erythrocytes from the immature cell cultures with high purity and in an efficient manner remains a significant challenge. A distinguishing feature of a reticulocyte and maturing erythrocyte is the increasing concentration of hemoglobin and decreasing cell volume that results in increased cell magnetophoretic mobility (MM) when exposed to high magnetic fields and gradients, under anoxic conditions. Taking advantage of these initial observations, we studied a noninvasive (label-free) magnetic separation and analysis process to enrich and identify cultured functional erythrocytes. In addition to the magnetic cell separation and cell motion analysis in the magnetic field, the cell cultures were characterized for cell sedimentation rate, cell volume distributions using differential interference microscopy, immunophenotyping (glycophorin A), hemoglobin concentration and shear-induced deformability (elongation index, EI, by ektacytometry) to test for mature erythrocyte attributes. A commercial, packed column high-gradient magnetic separator (HGMS) was used for magnetic separation. The magnetically enriched fraction comprised 80% of the maturing cells (predominantly reticulocytes) that showed near 70% overlap of EI with the reference cord blood-derived RBC and over 50% overlap with the adult donor RBCs. The results demonstrate feasibility of label-free magnetic enrichment of erythrocyte fraction of CD34+ progenitor-derived cultures based on the presence of paramagnetic hemoglobin in the maturing erythrocytes. © 2012 Jin et al

Parents’ journey through treatment for their child’s obesity: a qualitative study

Background: Treatment for childhood obesity is characterised by patient non-attendance and drop-out, and widespread failure to achieve weight maintenance. Qualitative methods may improve our understanding of patient perceptions and so improve treatment for childhood obesity. Aim: To provide insight into the perceptions of parents of obese children as they ‘journey’ from pre-treatment to end of treatment. Methods: We used purposive sampling and studied 17 parents of children attending 6-month outpatient treatments for obesity (BMI>98th percentile). Parent’s perceptions were explored by in-depth interviews, analysed using Framework methods. Results: Parents were characterised as being unaware of their child’s weight, in denial, or actively seeking treatment. Parents were consistently motivated to enter treatment due to perceived benefits to their child’s self esteem or quality of life, and weight outcomes appeared typically less important. During treatment parents expressed a lack of support for lifestyle changes outside the clinic, and noted that members of the extended family often undermined or failed to support lifestyle changes. Parents generally felt that treatment should have continued beyond six months, and it had provided benefits to their child’s well-being, self esteem, and quality of life, and this is what motivated many to remain engaged with treatment. Discussion: This study may help inform future treatments for childhood obesity by providing insights into the aspects of treatment of greatest importance to parents. Future treatments may need to consider providing greater support to lifestyle changes within the extended family, and may need to focus more on psycho-social outcomes

Piezoelectric Bolt Breakers and Bolt Fatigue Testers

A proposed family of devices for inducing fatigue in bolts in order to break the bolts would incorporate piezoelectric actuators into resonant fixtures as in ultrasonic/ sonic drills/corers and similar devices described in numerous prior NASA Tech Briefs articles. These devices were originally intended primarily for use as safer, more-reliable, more-versatile alternatives to explosive bolts heretofore used to fasten spacecraft structures that must subsequently be separated from each other quickly on command during flight. On Earth, these devices could be used for accelerated fatigue testing of bolts. Fatigue theory suggests that a bolt subjected to both a constant-amplitude dynamic (that is, oscillatory) stress and a static tensile stress below the ultimate strength of the bolt material will fail faster than will a bolt subjected to only the dynamic stress. This suggestion would be applied in a device of the proposed type. The device would be designed so that the device and the bolt to be fatigue-tested or broken would be integral parts of an assembly (see figure). The static tension in the tightened bolt would apply not only the clamping force to hold the joined structures (if any) together but also the compression necessary for proper operation of the piezoelectric actuators as parts of a resonant structural assembly. The constant-amplitude dynamic stress would be applied to the bolt by driving the piezoelectric actuators with a sinusoidal voltage at the resonance frequency of longitudinal vibration of the assembly. The amplitude of the excitation would be made large enough so that the vibration would induce fatigue in the bolt within an acceptably short time. In the spacecraft applications or in similar terrestrial structural-separation applications, devices of the proposed type would offer several advantages over explosive bolts: Unlike explosive bolts, the proposed devices would be reusable, could be tested before final use, and would not be subject to catastrophic misfire. In fatigue-testing applications, devices of the proposed type would offer advantages of compactness and low cost, relative to conventional fatigue- testing apparatuses. In both structural- separation and fatigue-testing applications, bolts to be broken or tested could be instrumented with additional ultrasonic transducers for monitoring of pertinent physical properties and of fatigue failure processes

Citizen science as a new tool in dog cognition research

The work of Á.M. was supported by the Hungarian Academy of Sciences (MTA 01 031).Family dogs and dog owners offer a potentially powerful way to conduct citizen science to answer questions about animal behavior that are difficult to answer with more conventional approaches. Here we evaluate the quality of the first data on dog cognition collected by citizen scientists using the Dognition. com website. We conducted analyses to understand if data generated by over 500 citizen scientists replicates internally and in comparison to previously published findings. Half of participants participated for free while the other half paid for access. The website provided each participant a temperament questionnaire and instructions on how to conduct a series of ten cognitive tests. Participation required internet access, a dog and some common household items. Participants could record their responses on any PC, tablet or smartphone from anywhere in the world and data were retained on servers. Results from citizen scientists and their dogs replicated a number of previously described phenomena from conventional lab-based research. There was little evidence that citizen scientists manipulated their results. To illustrate the potential uses of relatively large samples of citizen science data, we then used factor analysis to examine individual differences across the cognitive tasks. The data were best explained by multiple factors in support of the hypothesis that nonhumans, including dogs, can evolve multiple cognitive domains that vary independently. This analysis suggests that in the future, citizen scientists will generate useful datasets that test hypotheses and answer questions as a complement to conventional laboratory techniques used to study dog psychology.Publisher PDFPeer reviewe

Career Goals and Student Perceptions of a Post-Professional Occupational Therapy Doctoral Experiential Component

Clinical doctoral education for occupational therapy includes the acquisition of advanced clinical reasoning skills, leadership capacity, and opportunities for professional development and career achievement. Post-professional students represent motivated individuals seeking to enhance their education, experiences, and explore professional growth opportunities. While not required, the final semester of a clinical doctoral program typically includes a doctoral experiential component (DEC), or culminating project. The purpose of this retrospective, mixed-methods study was to examine the DEC goals and experiences of post-professional occupational therapy doctoral (POTD) students. A total of 49 student DEC proposals, each including multiple personal goals, were categorized according to one or more POTD program goals. The most frequently selected long-term DEC goals by participants in this study were developing educative roles (n = 37, 27.30%), scholarly activities (n = 31, 22.96%), and advanced clinical skills (n = 31, 22.96). The DEC goals developed by the students were also categorized according to the experience as perceived by the students. Three primary areas of growth experienced by the students during their DEC were: (1) improved self-awareness and confidence through reflection, (2) a challenging, yet fulfilling, experience and (3) translation of new knowledge and skills to practice. The outcomes of this study may offer insight for administrators and prospective students of post-professional programs and align with the American Occupational Therapy Association’s Vision 2025 to produce effective and collaborative leaders capable of implementing evidence-based, client-centered, and cost-effective treatment to influence the complexities of healthcare

A women’s worker in court: A more appropriate service for women defendants with mental health issues?

Aims Court liaison services aim to reduce mental illness in prison through early treatment and/or diversion into care of defendants negotiating their court proceedings. However, liaison services may inadvertently contribute to gender inequalities in mental health in the prison system. This is because women often do not access liaison services. This is attributed to services failing to recognise that women have different needs from men. To address this, it is essential that the needs of women in contact with the criminal justice system (CJS) are clearly articulated. However, there is a dearth of research that considers women’s needs at this stage of their journey through the CJS. This paper aims to identify these needs before women enter prison. It does so through an analysis of a pilot Women’s Support Service based at a Magistrates’ Court, a response to concerns that women were not accessing the local liaison service. Characteristics of women defendants attending the service are described, specifically their home environments, general and mental health needs. Their support needs when in contact with the CJS and the links the service must forge with local community organisations to provide this, are also presented. This knowledge will develop/ tailor existing services available to women defendants to improve their access to these and optimise the benefits they can derive from them. Methods Proformas were completed by a women specialist worker for 86 women defendants assessed in 4 months. Information was collected on characteristics including education, domestic violence, accommodation, physical and mental health.. This specialist worker recorded the range of needs identified by defendants at assessment and the services to which women were referred. Results Access to the Women’s Support Service is high, with only 11.3% of women refusing to use the service. Women attending have high levels of physical and mental health issues. Their mental health issues have not being addressed prior to accessing the service. Women often come from single households and environments high in domestic abuse. Women have multiple needs related to benefits, finance, housing, domestic abuse, education and career guidance. These are more frequent than those that explicitly link to mental health. The women’s worker providing the service referred women to 68 services from a wide variety of statutory and voluntary organisations. Conclusions The Women’s Support Service is accessed by a higher number of women, many more than access the local liaison service. It is suggested that this is due to their multiple and gender specific needs being adequately addressed by the former service and the organisations to whom they are referred. Mental health needs may also be secondary to other more basic needs, that makes the generic service provided but the Women’s support Service more appropriate than a liaison service that deals with mental health support alone

Cultural diversity and inclusion in UK medical schools

Background: Racially minoritised groups across the globe continue to experience differential outcomes in both health and education. Medical schools can play an instrumental role in addressing both these disparities, by creating inclusive student communities and ensuring that tomorrow's doctors can care for our increasingly diverse populations. Objectives: This collaborative, qualitative study led by three United Kingdom (UK) institutions aimed to explore the perspectives of Heads of Primary Care Teaching (HOTs) on cultural diversity and inclusion across UK medical schools. Methods: In December 2020, five focus groups were conducted remotely with 23 HOTs, or a nominated deputy. We explored participants' opinions regarding opportunities and barriers to cultural diversity and inclusion in medical education, ways to overcome these challenges and shared examples of best practice. Data were transcribed verbatim and thematically analysed by three researchers. Results: Investigators identified six themes from the data: lack of faculty diversity, tokenistic faculty training, institutional mindset, diversifying the formal and hidden curricula, intersectionality and student voice. Conclusion: Medical schools worldwide face similar challenges, uncertainties and opportunities when integrating diversity and inclusion throughout the learning environment. Although the importance of the topic is increasingly acknowledged, current efforts are viewed as being passive and tokenistic, hindered by challenges at multiple levels. Partnership with students and collaboration within and between institutions nationally and internationally will enable us to move forwards with both local and global positive, sustainable change